The use of environmental scanning electron microscopy (Aquasem) in coloproctology

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Klin Onkol 1999; 12(Suppl 1999): 37-40.

Summary: Aquasem is the environmental scanning electron microscope (ESEM) - a device which permits the direct observation, manipulation and analysis of many materials in their natural and unaltered state. The microscope operates under low vacuum and fresh or unfixed biological specimens are maintained wet in a separate chamber filled with water vapour. The aim of this study was to examine of small and large bowel and anal canal.
Different sample preparation methods were used in 34 speciemen and the results were compared with the use of both conventional scanning electron microscopy (SEM) and ESEM. In the future, we are to mine the tumor surfaces of colon, rectum and anus.

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