Konference: 2015 XI. Dny diagnostické, prediktivní a experimentální onkologie
Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie
Téma: Protinádorová léčiva a postupy II
Číslo abstraktu: 024
Autoři: Ing. Soňa Gurská, Ph.D.; Mgr. Pawel Znojek, Ph.D.; MUDr. Petr Džubák; doc. MUDr. Marián Hajdúch, Ph.D.
Introduction
The HTS (high throughput screening) is playing an important role in the early stage of drug development. This technique accelerates the screening of a large number of potential biological modulators against selected and specific targets. The aim of the screening processes is to select the bio-active compounds - hits.
Materials/methods
In the HTS robotic system, data processing and control software, liquid handling devices, and sensitive detectors are used in high complexity. The MTS test studying cell cytotoxicity is good example for demonstration of HTS capabilities where different cell lines and large compound libraries are used.
Results and conclusions
For selected active compounds the IC50 values are calculated. To quantify the suitability of the used cytotoxic assays in a HTS the Z-factor is determined for each plate and cell line. Obtained results, pros and cons of manual and robotic tests will be presented and discussed.
The research is supported by the National Sustainability Programme (LO1304).
Datum přednesení příspěvku: 3. 12. 2015
