Konference: 2009 5. sympózium a workshop molekulární patologie a histo-cyto-chemie
Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie
Téma: Selected presentations
Číslo abstraktu: 015
Autoři: Doc. MUDr. MVDr. Jozef Škarda, Ph.D. et Ph.D.; H. Grinberg-Rashi; E. Ofek; Marina Perelman; P. Yaron; J. Jacob-Hirsch; N. Kaminski; Ninette Amariglio; M. Krupsky; D.A. Simansky; I. Ram; D.M. Steinberg; R. Geletner; I. Ben-Dov; Z. Pfeffer
We have been screening genes encoding transmembrane/ secretory
proteins that are up-regulated in lung cancers and their brain
metastasis, with cDNA microarrays and tumor cells purified by
laser-capture microdissection. To verify the predictive value of
these gene products from the point of view of brain metastases, we
have been performing tumor tissue microarray analysis of clinical
lung cancer materials.
Preliminary results: RNA from 28 primary NSCLC, 8
samples of normal lung that were taken from the same patients,
seven independent brain metastasis and one specimen of normal brain
(commercial RNA that is derived from a pool of normal brains) were
hybridized to Affymetrix U95 Chips (containing 12625 probe sets).
Of the 28 primary NSCLC cases 6 developed brain metastases and 7
extra-cranial metastases during a minimal follow-up of three years.
Limited space precludes a detailed description of the analysis. The
microarray results were confirmed by qRT-PCR of selected genes.
ADAM8 and N-cadherin are according to these analysed genes
associated with brain metastasis in NSCLC patients screened above.
After verification on symplex from independent NSCLC patient files,
collected both in Israel and the Czech Republic, we found a
significant association between N-cadherin expression and brain
metastasis (p= 0.008).
Conclusion: N-cadherin is a very strong predictor
of brain metastasis in NSCLC patients.
Datum přednesení příspěvku: 24. 4. 2010
