Klin Onkol 2000; 13(Speciál2 2000): 49-54.
Summary: The study was designed to examine chemosensitivity of 12 established cell lines of malignant and normal origin on a panel of 7 cytostatics, and to explore the efficacy of 8 cytostatics on the primary cultures of malignant melanoma in in vitro conditions using the MTT assay. The rate of susceptibility of various cell lines to a damage induced by anticancer drugs was related to a histogenetic origin of cells and to the status of p53 gene. Our results showed that the effectiveness of DOX, and to a lesser extent of DDP, BLEO and VBL, was related to the histogenetic origin of cells with higher resistance of carcinoma than sarcoma cells. However, this association was not proved for 5-FU, MTX and TAX. The relationship between p53 gene alterations and the cell sensitivity to cytostatics was found only for 5-FU and VBL. Primary cultures of human malignant melanoma exerted an interesting feature. The cells partly grew in adherent phase and partly in suspension. Both of these forms gave positive reaction with three specific monoclonal antibodies (S100, HMB, NKI). The MTT assay was performed separately with each cellular fraction. With the exception of one primary culture showing high resistance to all drugs used the other tumour samples were markedly sensitive to VBL, DDP and DOX. Statistic analyses of MTT test results revealed higher resistance of melanoma cells growing in suspension comparing to adherent ones.
