Proliferation Activity in the Adult Rat Brain Following Exposure to Ionizing Radiation


Klin Onkol 2013; 26(5): 331-335. DOI: 10.14735/amko2013331.

Background: The aim of our study was to investigate radiation-induced short-term effects on the rat forebrain. Material and Methods: Adult male Wistar rats received whole- body exposure with fractionated doses of gamma rays (a total dose of 3 Gy) and were investigated seven and 14 days later. Immunohistochemistry and confocal microscopy were used to determine proliferating cells derived from anterior subventricular zone (SVZa) and distributed along the subventricular zone- olfactory bulb axis (SVZ- OB axis). Cell counting was performed in four anatomical parts along the well-defined pathway, known as the rostral migratory stream (RMS) represented by the SVZa, vertical arm, elbow and horizontal arm. Results: Different rate of cell overdistribution was found in all counted parts through the entire experiment, mostly detectable in the elbow and horizontal arm. Conclusion: Results suggested that radiation response of proliferating cells resides the SVZa may a play contributory role in the development of more adverse radiation-induced late effects.

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