Konference: 2009 5. sympózium a workshop molekulární patologie a histo-cyto-chemie
Nádorová biologie/imunologie/genetika a buněčná terapie
Číslo abstraktu: p009
Autoři: doc. MUDr. Tomáš Kučera, Ph.D.; H. Hroudová; Bashar Aldhoon; MUDr. Vojtěch Melenovský, CSc.; Prof. MUDr. Jindřich Martínek, DrSc.; Prof. MUDr. Josef Kautzner, CSc., FESC
Atrial fibrillation is a common disorder and its
relation to myocardial fibrosis is currently under investigation.
The aim of our study was to evaluate the level of fibrosis
manifested as collagen volume fraction (CVF), together with the
immunohistochemical detection of transforming growth factor-beta
(TGF-beta) and connective tissue growth factor (CTGF) in patients
suffering from end stage heart failure with or without atrial
fibrillation. TGF-beta and CTGF are involved in fibrotic processes
in various organs and their role in myocardial fibrosis has been
proposed, as well. The study was performed in two groups of
patients - one group with atrial fibrillation and one group with
sinus rhythm. In both of these groups were patients indicated for
heart transplantation, who were diagnosed with ischemic heart
disease or cardiomyopathy. The atrial and the ventricular samples
were fixed with paraformaldehyde and embedded into paraffin.
Histochemical detection of collagen for quantification of collagen
volume fraction (CVF) was performed using Sirius red staining.
Immunohistochemical detection of TGF-beta and CTGF was performed
using three-step immunoperoxidase reaction. Image analysis software
was used for the quantitation of CVF and immunohistochemical
reaction product. When the CVF was quantified in the right atrial
myocardium and in the left ventricular myocardium, there was
nosignificant difference in CVF between the atrial fibrillation and
the sinus rhythm group. CTGF was detected in all samples obtained
from both groups of patients. It was constantly expressed in
cardiomyo-cytes. TGF-beta was also detected in all samples.
However, its expression in atria was not as widespread as that of
CTGF. TGF-beta was found mainly in the endothelial lining of atria
and in the capillary endothelium. Cardiomyocytes expressed TGF-beta
in some of the samples; mainly in regions adjacent to endocardium.
TGF-beta was more abundant in ventricles than in atria. In summary,
we found that in patients with end stage heart failure there was no
difference in CVF between groups of patients with atrial
fibrillation and with sinus rhythm. In addition, we detected both
CTGF and TGF-beta in atria and ventricles of patients from these
Supported by MSMT Grant No. 0021620807.
Datum přednesení příspěvku: 24. 4. 2009