Identification of bio-active molecules in HTS

Konference: 2015 XI. Dny diagnostické, prediktivní a experimentální onkologie

Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie

Téma: Protinádorová léčiva a postupy II

Číslo abstraktu: 024

Autoři: Ing. Soňa Gurská, Ph.D.; Mgr. Pawel Znojek, Ph.D.; MUDr. Petr Džubák; doc. MUDr. Marián Hajdúch, Ph.D.


The HTS (high throughput screening) is playing an important role in the early stage of drug development. This technique accelerates the screening of a large number of potential biological modulators against selected and specific targets. The aim of the screening processes is to select the bio-active compounds - hits.


In the HTS robotic system, data processing and control software, liquid handling devices, and sensitive detectors are used in high complexity. The MTS test studying cell cytotoxicity is good example for demonstration of HTS capabilities where different cell lines and large compound libraries are used.

Results and conclusions

For selected active compounds the IC50 values are calculated. To quantify the suitability of the used cytotoxic assays in a HTS the Z-factor is determined for each plate and cell line. Obtained results, pros and cons of manual and robotic tests will be presented and discussed.

The research is supported by the National Sustainability Programme (LO1304).

Datum přednesení příspěvku: 3. 12. 2015