Immunohistochemical analysis of KLK11, KLK7, PSA and PSMA in cancerous and noncancerous prostatic tissues

Konference: 2010 6. sympózium a workshop molekulární patologie a histo-cyto-chemie

Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie

Téma: Postery

Číslo abstraktu: p006

Autoři: T. Jamaspishvili; MUDr. Milan Král, Ph.D.; I. Khomeriki; D. Kurfürstová; Mgr. Jan Bouchal

Introduction and aims: Kallikreins (KLK) 7 and 11 share a high degree of structural similarity with PSA and other KLKs and may serve as new biomarkers for prostate cancer. The aim of this study was to evaluate differences of KLK11/KLK7 expression in the paired cancer/benign prostate foci of the same patient and to define any association with clinicopathological parameters.

Methods: Seventy archived paraffin-embedded tissues obtained from radical prostatectomy were used for immunohistochemical analysis (IHC) and stained for KLK7, KLK11, PSA and PSMA. Intensity of IHC staining and percentage of positive tumor cells were determined for both BPH (benign prostatic hyperplasia) and CaP (prostate carcinoma) foci in each patient. Statistical analysis was done with SPSS using Wilcoxon, Spearman and Kruskal-Wallis non-parametric tests.

Results: We found significant differences for all studied proteins between BPH and CaP foci. Both KLK7 (p=0.026) and KLK11 (p<0.001) expressions were decreased in prostate cancer compared with its benign counterparts. The same difference was found for PSA (p<0.001) while reverse one for PSMA expression (p<0.001). Positive correlations were found for both KLK7 (Rs=0.74) and KLK11 (Rs=0.35) in CaP and BPH. No correlation or significant differences were found with respect to Gleason score, tumor grade, pT stage or serum PSA values. We only found slight upregulation of KLK11 in advanced cases when compared with localized ones (p=0.026).

Conclusion: For the first time, we report lower IHC expression of KLK11 in CaP than in BPH and also its slight upregulation in advanced tumors accord compared to localized ones. In concordance with the literature, we confirm both higher expression of PSMA and lower expression of KLK7 and PSA in CaP compared with BPH.

This work was supported by grants NS 9940-4 and MSM 6198959216. Tamara Jamaspishvili was also supported by GACR 303/09/H048.

Datum přednesení příspěvku: 23. 4. 2010