N-cadherin as a potential predictor of metastases to central nervous system in NSCLC

Konference: 2009 5. sympózium a workshop molekulární patologie a histo-cyto-chemie

Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie

Téma: Selected presentations

Číslo abstraktu: 015

Autoři: MUDr. MVDr. Jozef Škarda, Ph.D.; H. Grinberg-Rashi; E. Ofek; Marina Perelman; P. Yaron; J. Jacob-Hirsch; N. Kaminski; Ninette Amariglio; M. Krupsky; D.A. Simansky; I. Ram; D.M. Steinberg; R. Geletner; I. Ben-Dov; Z. Pfeffer

We have been screening genes encoding transmembrane/ secretory proteins that are up-regulated in lung cancers and their brain metastasis, with cDNA microarrays and tumor cells purified by laser-capture microdissection. To verify the predictive value of these gene products from the point of view of brain metastases, we have been performing tumor tissue microarray analysis of clinical lung cancer materials.

Preliminary results: RNA from 28 primary NSCLC, 8 samples of normal lung that were taken from the same patients, seven independent brain metastasis and one specimen of normal brain (commercial RNA that is derived from a pool of normal brains) were hybridized to Affymetrix U95 Chips (containing 12625 probe sets). Of the 28 primary NSCLC cases 6 developed brain metastases and 7 extra-cranial metastases during a minimal follow-up of three years. Limited space precludes a detailed description of the analysis. The microarray results were confirmed by qRT-PCR of selected genes. ADAM8 and N-cadherin are according to these analysed genes associated with brain metastasis in NSCLC patients screened above. After verification on symplex from independent NSCLC patient files, collected both in Israel and the Czech Republic, we found a significant association between N-cadherin expression and brain metastasis (p= 0.008).

Conclusion: N-cadherin is a very strong predictor of brain metastasis in NSCLC patients.

Datum přednesení příspěvku: 24. 4. 2010