Analysis of the prognostic impact of nestin expression in non-small cell lung cancer

Konference: 2010 6. sympózium a workshop molekulární patologie a histo-cyto-chemie

Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie

Téma: Postery

Číslo abstraktu: p010

Autoři: Doc. MUDr. MVDr. Jozef Škarda, Ph.D.; prof. MUDr. Zdeněk Kolář, CSc.; Mgr. Mária Janíková; MUDr. Jana Chmelová; Mgr. Veronika Krejčí; Mgr. Jana Zapletalová; Mgr. Kateřina Langová, Ph.D.; prof., MUDr. Jiří Klein, Ph.D., PETCS; MUDr. Ivona Grygárková; prof. MUDr. Vítězslav Kolek, DrSc.; Dr. Eitan Fridman; Juri Kopolovic

Background: Nestin expression in tumor cells may be associated with the degree of tumor differentiation, biological potential of tumor and degree of neoangiogenesis.

Methods: Immunohistochemical detection of nestin was performed by indirect immunohistochemistry using available anti-bodies on tissue microarrays constructed from 115 formalin-fixed paraffin-embedded non-small cell lung cancer samples and 35 brain metastasis. The H-scoreand degree of nestin positive vascularisation evaluated semi-quantitatively in areas of the most prominent vascularisation were determined. The parameters were correlated with each other and with disease-free and overall survival.

Results: Significantly higher expression of nestin was found in brain metastases of squamous cell carcinomas compared to brain metastases of adenocarcinomas (p=0.003). In sqamous cell carcinomas and adenocarcinomas a significantly higher expression of nestin was found in brain metastases compared to primary tumors (p<0.0001, p=0.034). There was significantly higher occurrence of nestin positive vascularisation in brain metastases of adenocarcinomas than primary adenocarcinomas (p=0.044). In brain metastasis, there was significantly higher occurrence of nestin positive vascularisation in poorly differentiated adenocarcinomas than in well-differentiated adenocarcinomas (p=0.927) or poorly differentiated primary adenocarcinomas (p = 0.008).

Kaplan-Meier curves of disease-free and overall survival showed no significant correlation with nestin expression except for better overall survival of patients with brain metastasis and high nestin expression than patients with brain metastasis and low nestin expression (p=0,043).

Conclusion(s): Our results suggest that the immunohistochemical expression of nestin may be an important diagnostic marker

Datum přednesení příspěvku: 23. 4. 2010