Kategorie: Onkologická diagnostika
Téma: Keynote lectures of invited speakers II
Číslo abstraktu: 011
Technology & Method: The TissueFAXS system consists of a high-end motorized fluorescence microscope and sophisticated software for image analysis. The samples can be stained by means of immunohistochemistry or immunofluorescence. By using the nuclear marker (e.g. hematoxilin or DAPI) the location of the individual cells is identified and the systém quantifies the staining intensity in each color channel for each and every cell. It allows even to distinguish between and compare between nuclear and cytoplasmatic marker expression in the same sample. For each cell or cellular compartment up to 14 parameters are measured and stored in a database. Representation of cellular parameters may be obtained by dot plots and/or histograms in a FACS-like manner.
Results: Results presented come from several studies and comprise different applications of slidebased cytometry.(i) Phenotypic characterization of tissue infiltrating leukocytes in tumor biology(1), transplantation immunology(2) and autoimmune diseases(3) exemplifying the quantification of markers CD1a, CD3, CD4, CD8, CD11c, and CD68 in renal cell carcinoma, renal allograft rejection and atopic dermatitis. (ii) Signal transduction research in prostate cancer(4,5) and brain neuropathology(6) dealing with signal transducers and activators of transcription (STAT), and transforming growth factor beta. (iii) Quantification of proliferation marker Ki67 and Her2 in breast cancer.
Discussion: The data above demonstrate novel approaches in research and improved data transparency in diagnosis using slide-based cytometry. Observer-biased visual estimation in immunohistological analysis of tissue samples is replaced by observer independent measurements on the single-cell level, which is especially important in multi-center studies and set-up of clinical studies for the pharmaceutical industry.
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Datum přednesení příspěvku: 29. 4. 2011