Číslo abstraktu: p015
Methods: Breast carcinomas were investigated by imuno-histochemistry (IHC), fluorescence in situ hybridisation (FISH) and comparative genomic hybridisation (CGH).
Results: IHC was performed with DAKO Cytomation HercepTest and FISH with PathVysion Probe Kit. A total of 213 (10,56%) of 2017 patients were positive by IHC. These positive IHC results (2+, 3+) were verified by FISH. HER2/NEU gene amplification was confirmed in 138 cases (70%). CGH was performed at 29 cases to screen breast tumors for copy number changes. We obtained successful results in 26 of 29 cases. 22 cases were positive for copy number changes, 4 cases were negative for copy number changes probably due to analysis of non tumour tissue. The most frequent DNA sequence copy number changes were gains of 1q (14 cases), 8q (13 cases) and loss of 1p (14 cases), 16q (12 cases).
Conclusions: Our results indicate that especially borderline results of IHC (2+) should be interpreted with caution using both IHC and FISH with standardised metodology. The prevalence of the most common copy number aberrations detected by CGH was roughly similar to that reported in the literature.
Datum přednesení příspěvku: 24. 4. 2009