Konference: 2010 6. sympózium a workshop molekulární patologie a histo-cyto-chemie
Kategorie: Nádorová biologie/imunologie/genetika a buněčná terapie
Téma: Workshop on Molecular Pathology
Číslo abstraktu: w003
Autoři: Andrea Staršíchová; Eva Lincová; Mgr. Zuzana Pernicová; prof. RNDr. Alois Kozubík, CSc.; Mgr. Karel Souček, Ph.D.
Chronic inflammation plays an important role in
initiation and progression of various human diseases including
benign prostatic hyperplasia or prostate cancer. Here, we show that
proinflammatory cytokine interleukin-6 (IL-6) has prosurvival
effects and chronically activates Jak2/STAT3 signaling pathway in a
model of benign prostatic hyperplasia. We demonstrate that
anti-inflammatory cytokine transforming growth factor ßi (TGF-ß1),
which also permanently activates its canonical signaling pathway
through SMAD proteins in BPH-1 cells, affects the effects of IL-6
on cell proliferation. Moreover and importantly, TGF-ß1 inhibits
IL-6 signal transduction (during longtime action of both cytokines)
by decreasing phosphorylation levels of STAT3 and decreases nuclear
localization of phosphorylated STAT3 as well. This is associated
with reduced expression of Jak2 at both mRNA and protein levels. In
conclusion, our data show that TGF-ß1 reverts prosurvival effects
of IL-6 through regulation of Jak2 expression in prostate
epithelial cells.
Datum přednesení příspěvku: 24. 4. 2010
