THE POTENTIAL ROLE OF CIRCULATING TUMOR CELLS/DISSEMINATED TUMOR CELLS DETECTED BY EGFR GENE EXPRESSION IN RESECTABLE NON-SMALL CELL LUNG CANCER PATIENTS

Konference: 2016 XL. Brněnské onkologické dny a XXX. Konference pro nelékařské zdravotnické pracovníky

Kategorie: Zhoubné nádory plic a průdušek

Téma: XXIII. Nádory plic, průdušek a pleury

Číslo abstraktu: XXIII/175

Autoři: Alona Chasovskykh; Mgr. Andrea Prokopová; Bc. Monika Vahalíková; prof., MUDr. Jiří Klein, Ph.D., PETCS; MUDr. Josef Chudáček; MUDr. Marek Szkorupa; MUDr. Ivona Grygárková; Mgr. Jana Vrbková, Ph.D.; MUDr. Josef Srovnal, Ph.D.; doc. MUDr. Marián Hajdúch, Ph.D.

 Background:

Based on the World Health Organization data, lung cancer hold a long time a leading position of cancer mortality in the world. Lung cancer counted 1.59 million cases of death, among the 8.2 million deaths from malignant tumours. Consequently, the development of prognostic and predictive biomarkers for non-small cell lung cancer (NSCLC) is an important issue. Therefore, analysis of circulating tumor cells/disseminated tumor cells (CTCs/DTCs) has recently received a lot of interest for this purpose. The literature has accumulated evidence showing that the CTCs may be considered as an independent prognostic parameter of cancer progression in breast, colorectal and prostate cancer. The aim of our study was to analyse prognostic value of CTCs using EGFR expression in NSCLC patients.

Materials and Methods:

A total of 119 patients with IA-IIIA stage NSCLC (85 men and 34 women), were enrolled in the study. Pulmonary venous blood, bone marrow aspirate and two samples of peripheral venous blood (before surgery and one month after surgery) were collected from each patients. CTCs/DTCs were detected by RT-PCR real time using Rotor Gene 3000. The number of epidermal growth factor receptor (EGFR) copies was counted and cut-off gene expression values was determined for showing the CTCs/DTCs presence/absence. The data were compared with clinical and morphological characteristics and their impact on overall survival (OS), cancer-specific survival (CSS) and disease-free survival (DFS) was evaluated by Kaplan-Meier method.

Results:

For the threshold values determination, samples from 12 patients with non-cancerous diagnosis (hernias and cholecystolithiasis) and from 58 healthy blood donors were analysed. The cut-offs values were set at 250 EGFR RNA copies per microgram of total RNA for peripheral or pulmonary blood and 30,000 per microgram of total RNA for bone marrow. According to the above threshold values, CTCs/DTCs were positive in 23.5% of peripheral blood samples, in 42.8% of tumor-draining blood samples and in 49.6% of bone marrow samples. The EGFR gene expression has no statistically significant impact on OS, CSS and DFS.

This work was financially supported by Ministry of Health of the Czech Republic, grant No. 15-29021A, IGA UP LF 2016_010 and NPU LQ1304.

 

Datum přednesení příspěvku: 28. 4. 2016