Téma: Selected presentations
Číslo abstraktu: 014
Aim: This study reports on marker profiling changes of normal keratinocytes under the influence of mesenchymal cells derived from basal cell carcinoma in vitro, and especially on the induction of stem-cell-like features in affected epithelia. Material and methods: Weperformed a genomic search for up/ down-regulated genes for growth factors in stromal fibroblasts. We compared the phenotype of human tumors with an in vitro model where growth and phenotypic pattern of normal human keratinocytes were monitored under the influence of various types of fibroblasts including those prepared from stroma of basal cell carcinomas.
Results: We visualized a panel of keratins, endogenous lectins and binding sites for endogenous lectins, and markers of stem cells and growth factors which were upregulated. The observed epithelial cells in coculture subsequently expressed tumor markers as well as some putative stem cell markers.
Conclusion: Epithelial-mesenchymal interactions are of crucial importance in the course of malignant tumors, where stroma influence the behavior of the transformed epithelial cells. This biological activity can be attributed to the production of soluble paracrine factors and can be blocked by proper monoclonal antibodies. This observation can be of a great future therapeutical importance.
This study was supported by Ministry of Education Youth and Sport of the Czech Republic, projects No. MSM0021620806 and No. 1M0538.
Datum přednesení příspěvku: 24. 4. 2009