Konference: 2006 2. ročník Dny diagnostické, prediktivní a experimentální onkologie
Číslo abstraktu: 023p
Autoři: MUDr. Ingrid Garajová; MUDr. Pavel Fabián, Ph.D.; MUDr. Rudolf Nenutil, CSc.; doc. MUDr. Ilona Kocáková, Ph.D.; MUDr. Peter Grell; Zina Hanzelková; Ing. Dana Knoflíčková; prof. MUDr. Marek Svoboda, Ph.D.; prof. MUDr. Rostislav Vyzula, CSc.
Tumor is a complex tissue composed of cancer
cells and stromal cells (e.g. endothelial cells, fibroblasts,
dendritic and NK cells, macrophages and lymphocytes).
Tumor-infiltrating lymphocytes (TIL) are found in a variety of
solid cancers and they are a possible prognostic factor as it is
thought that TILs execute a host immune response against cancer
cells. In colorectal carcinoma (CRC), TILS are particularly
numerous in cases associated with microsatellite instability and
have more favorable clinical outcome. Generally, cytotoxic T-cells
(CD8+) are prognostically favorable, whereas recent discovered
subgroup of TILs, regulatory T-cells (T-reg,CD4+CD25+FOXP3+) are
not. They inhibit antitumor activity of CD8+ and CD4+ T-cells. The
aim of our study was to investigate if the TIL of CRC include T-reg
lymphocytes, which was not proved so far. More recent studies have
shown that T-reg lymphocytes are unically characterized by
expression of transcription factor FOXP3. Therefore we used
immunohistochemical staining to detect lymphocytes co-expressing
CD4+ and FOXP3+ in 9 cases of CRC primary tumors. All cases of CRC
were left-side localized, respecting a different biological
behaviour of left/right-side localized CRCs. We used formalinfixed
and paraffin-embedded sections and commercially available
monoclonal antibodies. Our preliminary results show that TIL in CRC
include in cancer stroma a subset of CD4+ CD25+FOXP3+ lymphocytes.
Now, we are interested if T-reg lymphocytes can be used as a
prognostic marker for CRC and we analyze a group of 20 patients
with CRC in I-IV clinical stage. We are also interested if there is
a connection between occurrence of T-reg and other stromal cells,
especially cytotoxic T-lymphocytes and NK cells.
Acknowledgment: This project is
supported by Internal Grant Agency, Ministry of Health, Czech
Republic. No.: NR/9076-4.
Datum přednesení příspěvku: 7. 12. 2006