Kategorie: Maligní melanom a nádory kůže
Téma: V. Monitorování a predikce v onkologii
Číslo abstraktu: 045
Autoři: Prof. MUDr. Jaroslav Michálek, Ph.D.; doc. MUDr. Vuk Fait, CSc.; MUDr. Ivo Kocák, Ph.D.; Petra Vidláková; Mgr. Lucie Říhová, PhD.; prof. MUDr. Jan Žaloudík, CSc.; prof. MUDr. Roman Hájek, CSc.; Ellen S. Vitetta, Ph.D.
We investigated the presence of individual melanoma-specific T
cell clones in patients with metastatic melanoma. Ten patients were
examined for the presence of melanoma-reactive T cells using
dendritic cells loaded with autologous tumor cells. Their
specificity was tested using non-radiactive cytotoxicity test.
Individual immunodominant T cell clones were identified by the
clonotypic assay that combines in vitro cell culture,
immunomagnetic sorting of activated IFNg+ T cells, T cell receptor
beta locus anchored reverse transcription-PCR, and clonotypic
All patients had detectable melanoma-reactive T cells in vitro. Expanded melanoma-reactive T cells demonstrated specific cytotoxic effect against autologous tumor cells in vitro. Three patients experienced objective responses and their clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8+ T cell clones with frequencies of 10–6 to 10–3 of all circulating CD8+ T cells. Five patients with progressive disease experienced no or temporary presence of circulating melanoma-reactive T cell clones. Thus, circulating immunodominant CD8+ T cell clones closely correlate with clinical outcome in patients with metastatic melanoma.
Supported by MŠMT NPVII 2B06058.
Datum přednesení příspěvku: 25. 4. 2007