MicroRNA Assessment as a New Diagnostic and Prognostic Tool of Barrett´s Esophagus. Pilot Study

Konference: 2011 7. Sympozium a workshop molekulární patologie a histo-cyto-chemie

Kategorie: Onkologická diagnostika

Téma: Postery

Číslo abstraktu: 009p

Autoři: Mgr. Pavla Lužná; MUDr. Jan Gregar; Ing. Ivo Überall, Ph.D.; Doc. MUDr. Vlastimil Procházka, Ph.D.; Prof. MUDr. Jiří Ehrmann jr., Ph.D.

Backround: Barrett‘s esophagus (BE) is the disease which can progress through several stages into the adenocarcinoma of the esophagus (EAC). Since 1993 the process of carcinogenesis has been investigated in connection with regulators of gene translation – microRNAs. It was found that among them miR-21, miR-192, miR-196a and miR-203 were often related to progression and prognosis of various tumors. Therefore, the aim of this pilot study was to assess the role of these miRNA in BE in various stages of progression.

Design: 30 patients diagnosed with BE (15 cases without dysplasia, 10 cases with low grade dysplasia and 5 cases with high grade dysplasia/adenocarcinoma) were examined. 5 cases of normal esophagus were used as a control. Samples from paraffin blocks were cut into the slides, specific lesions were microdissected, total RNA was isolated and microRNA assays were carried out. Their levels were relatively quantified to two endogenous controls RNU44 and RNU48.

Results: We found significant increase of miR-192 levels in all stages of BE compared to the control. The levels of miR-203 were downregulated in all cases and moreover negatively correlated with the progression of disease. miR-21 expression was increased in all cases without dysplasia and in all cases with low grade dysplasia, however in cases with high grade dysplasia/adenocarcinoma it was not changed. Conversely, miR-196a was amplified in all adenocarcinoma cases and not in dysplastic lesions.

Conclusion: Our results suggest that detection of miR-192 may be important for primary diagnosis of Barrett‘s esophagus. Moreover, we found out that detection of miR-203, miR-21 and miR-196a may play role in assessment of progression of BE. Therefore, it seems that miRNA assays may serve as a new diagnostic and prognostic tool for BE.

Acknowledgement: This work was supported by Grant of Ministry of Health of Czech Republic, No. NS 10279-3 and by Grant MSM 6198959216.

Datum přednesení příspěvku: 29. 4. 2011