Konference: 2011 7. Sympozium a workshop molekulární patologie a histo-cyto-chemie
Kategorie: Onkologická diagnostika
Téma: Postery
Číslo abstraktu: 009p
Autoři: Mgr. Pavla Lužná; MUDr. Jan Gregar; Ing. Ivo Überall, Ph.D.; Doc. MUDr. Vlastimil Procházka, Ph.D.; Prof. MUDr. Jiří Ehrmann jr., Ph.D.
Backround: Barrett‘s esophagus (BE) is the
disease which can progress through several stages into the
adenocarcinoma of the esophagus (EAC). Since 1993 the process of
carcinogenesis has been investigated in connection with regulators
of gene translation – microRNAs. It was found that among them
miR-21, miR-192, miR-196a and miR-203 were often related to
progression and prognosis of various tumors. Therefore, the aim of
this pilot study was to assess the role of these miRNA in BE in
various stages of progression.
Design: 30 patients diagnosed with BE (15 cases
without dysplasia, 10 cases with low grade dysplasia and 5 cases
with high grade dysplasia/adenocarcinoma) were examined. 5 cases of
normal esophagus were used as a control. Samples from paraffin
blocks were cut into the slides, specific lesions were
microdissected, total RNA was isolated and microRNA assays were
carried out. Their levels were relatively quantified to two
endogenous controls RNU44 and RNU48.
Results: We found significant increase of miR-192
levels in all stages of BE compared to the control. The levels of
miR-203 were downregulated in all cases and moreover negatively
correlated with the progression of disease. miR-21 expression was
increased in all cases without dysplasia and in all cases with low
grade dysplasia, however in cases with high grade
dysplasia/adenocarcinoma it was not changed. Conversely, miR-196a
was amplified in all adenocarcinoma cases and not in dysplastic
lesions.
Conclusion: Our results suggest that detection of
miR-192 may be important for primary diagnosis of Barrett‘s
esophagus. Moreover, we found out that detection of miR-203, miR-21
and miR-196a may play role in assessment of progression of BE.
Therefore, it seems that miRNA assays may serve as a new diagnostic
and prognostic tool for BE.
Acknowledgement: This work was supported by
Grant of Ministry of Health of Czech Republic, No. NS 10279-3 and
by Grant MSM 6198959216.
Datum přednesení příspěvku: 29. 4. 2011
