Konference: 2006 48th ASH Annual Meeting - účast ČR
Kategorie: Mnohočetný myelom
Téma: Publikace ve sborníku
Číslo abstraktu: 5012
Autoři: prof. Ing. Kyra Michalová, DrSc.; Doc.RNDr. Zuzana Zemanová, CSc.; Mgr. Lenka Pavlištová, Ph.D.; Mgr. Jana Skuhrovcová - Tajtlová; Prof.MUDr. Ivan Špička, PhD; René Zounar; MUDr. Evžen Gregora; Alena Smolikova; Michal Chrz; MUDr. Jarmila Obernauerová; MUDr. Lenka Walterová
During the last two years we examined 128 newly diagnosed patients with MM by conventional G-banding technique and by I-FISH. We focused on detection of aberrations of 13q, IgH gene rearrangements, and t(11;14)(q13;q32) translocation. I-FISH was done by locus-specific DNA probes (Abbott-Vysis, Des Plaines, Illinois, USA). Fifteen patients with complex karyotype were re-examined by multicolor FISH (mFISH, MetaSystems GmbH, Altlussheim, Germany). G-banding revealed abnormal karyotypes in 25% of patients, I-FISH detected chromosomal abberations in 82.8% cases. Abberations of chromosome 13 were found in plasma cells of 84 patients (65,6%), the incidence was: del(13)(q14) in 11%, monosomy 13 in 40%, combination of del(13)(q14)/monosomy 13 in 8%, and other aberrations of chromosome 13 in 7% of cases. Aberrations of IgH gene were proved in 82 patients (64%). t(11;14)(q13;q32) was seen in fourteen patients (11%) and other translocations affecting 14q32 region in 19 patients (15%). Besides translocations, different variations of total and/or partial deletions of IgH gene were detected in 28 cases (22%). In nine patients (7%) we found clones with translocation and deletion of 14q32 simultaneously, twelve patients (9%) had other IgH aberrations. Univariate statistical testing showed aberrations of 13q (p=0.042), and IgH gene rearrangments (translocations except t(11;14)(q13;q32) or deletions; p=0.007) to be associated with significantly shorter progression-free survival. The worst prognosis in our cohort was for 18 patients with combination of chromosome 13 anomalies together with rearrangements of IgH gene (p=0.049). Method of immunofluorescent labeling of plasma cells allows hIgHer detection of chromosomal changes by I-FISH technique and therefore correct prognosis of the disease can be done.
Grant support: MZO 00064165, IGA NR/8183-4 and MSM 0021620808.
Datum přednesení příspěvku: 9. 12. 2006